DOSIS-3D: The crew retrieved the DOSIS-3D Passive Detector Pouch (PDP) from the Dragon vehicle and installed the ten passive detector packs and one triple detector pack at appropriate locations. This experiment uses active and passive detectors to determine the radiation doses inside the ISS and provides documentation of the actual nature and distribution of the radiation fields. A concise three dimensional dose distribution map of all the segments of the ISS will be developed based on this data and data from JAXA and NASA monitoring devices.
Engineered Heart Tissue/FE8: Using the Life Science Glovebox, the crew performed the cell culture media change for the Engineered Heart Tissue chambers. A Human induced Pluripotent Stem Cell (iPSC)-based 3D Microphysiological System for Modeling Cardiac Dysfunction in Microgravity (Engineered Heart Tissues) assesses human cardiac function in microgravity. It uses 3D cultured cardiac muscle tissue that mimics the architecture and function of adult human cardiac muscle. A magnet-based sensor placed underneath the tissue culture chamber allows real-time, non-destructive analysis of the functional performance and maturation of the tissues in space, which is compared with those cultured on Earth.
Medium Temp PCG: The crew retrieved the JAXA Protein Crystal Growth (PCG) container, started the investigation, then stowed it in Freezer-Refrigerator Of STirling cycle (FROST2). The high quality protein crystal growth experiment at moderate temperature (JAXA MT PCG) is aimed at the growth of crystals of biological macromolecules by the counter-diffusion technique or permeation method. The main scientific objective of the JAXA Moderate Temp PCG experiment is to make high quality protein crystals in the microgravity environment at moderate temperature.
Nanoracks Mod-51: The crew initiated the sub investigations contained within the Mod-51. These sub experiments are the Vitamin-C Flask with syringe, Waxworm flask, Adipic acid and Venom flex plates, Yeast and amylase mixture tubes, and Flask with Flatworms. Inspection and/or activation of the experiments were nominal and the hardware was stowed. Nanoracks Mod-51 is an aluminum module with a Velcro lid that can accommodate various sub-experiments.
OsteoOmics-2: The crew completed media exchange for Biocell habitat 4. During the activity, they noted only 3 of the biocells were free of contamination. The media was exchanged on the 3 healthy Biocells and the 3 Biocells with contamination were bagged and frozen for later ground analysis. Cause of the contamination is under discussion. Millions of Americans experience bone loss which results from disease or the reduced effects of gravity that can occur in bed-ridden patients. OsteoOmics tests whether magnetic levitation accurately simulates the free-fall conditions of microgravity by comparing genetic expression osteoblastic cells, a type of bone cell, levitated in a high-field superconducting magnet with cells flown in low-Earth orbit. This information helps scientists determine the molecular and metabolic changes that take place in magnetic levitation and real microgravity.
PCG-10: The crew retrieved the PCG-10 hardware from its temporary ambient stow location and placed it in the permanent stow location. Microgravity Crystallization of Glycogen Synthase-Glycogenin Protein Complex (CASIS PCG 10) crystallizes human glycogen synthase proteins on the space station. These include enzymes critical for glycogen synthesis in the liver and those present in muscle. The lack of convection in microgravity can result in higher quality crystals that contribute to better understanding of a protein’s structure and support development of more effective medications with fewer side effects.
Systems
Latching End Effector (LEE) A Snare Survey: Following yesterday’s Space Station Remote Manipulator stem (SSRMS double walkoff, today the LEE A Snare survey was completed. The Robotics team will now unstow the Special Purpose Dexterous manipulator (SPDM) and Robot Micro Conical Tool (RMCT) in preparation for tomorrow’s Material International Space Station Experiment (MISSE) operations.
Completed Task List Activities:
- None
Today’s Ground Activities:
All activities are complete unless otherwise noted.
- Payload operations support
- SSC updates
- SSRMS LEE A survey
Lookahead Plan
Thursday, 3/12 (GMT 072)
Payloads:
- Food Acceptability
- Locker reconfigurations
- MELFI Icebrick insert
- MISSE/JEMAL ops
- Nanoracks Mod-9 ops 2
Systems:
- JOTI config for MISSE transfer tray
Friday, 3/13 (GMT 073)
Payloads:
- Food Acceptability
- Standard Measures
- MVP2-cell-03
- OsteoOmics
- Phage Evolution
Systems:
- WHC maintenance
Saturday, 3/14 (GMT 074)
Payloads:
- CGBA stat check
- ISS Experience SD c/o
- MELFI icebrick insertions
- MVP-Cell-03
- Nanoracks Mod-51
Systems:
- Crew off duty; housekeeping
Sunday, 3/15 (GMT 075)
Payloads:
- None
Systems:
- Crew off duty
Today’s Planned Activities:
All activities are complete unless otherwise noted.
- HRF Generic MELFI Sample Retrieval And Insertion Operations
- NanoRacks Module-51 Overview
- Nanoracks Module-51 MELFI Retrieve
- OsteoOmics-2 Media Relocate
- MERLIN 4 EXPRESS Rack Installation
- Polar and PAUL Transfer from Dragon Overview
- Cold Stow Box Unpack
- XF305 Camcorder Setup
- Weekly check of video recording equipment performance on the ISS RS
- JAXA MT PCG Sample Preparation
- AED Inspection
- RSK1 Laptop Replacement
- OsteoOmics-2 Media Change
- LSG-Front Glove-Swap
- Fabrication and installation of improved ventilation aids for RSK1 ZBook laptop
- EHT MELFI Sample Retrieval Operation 1
- Nanoracks Module-51 Start
- VIZIR. Conducting Smart Drawer Coordinate Referencing System (СКП-УП) PL Session
- MELFI 3 Ice Brick Insert 2
- JAXA Mouse Mission 5 Item Gathering and Preparation
- TOCA Water Recovery System (WRS) Sample Analysis
- XF305 Camcorder Setup
- Transportation Cage Unit Cleaning
- MERLIN Sample Transfer
- Polar and PAUL Transfer from Dragon Overview
- Setup and Activation of Crew Onboard Support Kit (КСПЭ) Equipment for mpeg2 TV coverage of Wide Band Communication System (ШСС) Test
- Glove Box Cleaning
- Gathering and connecting КСПЭ hardware to ШСС video server
- Polar Dragon Uninstall, Transfer, and EXPRESS Rack Install
- Test comm sessions via Luch-5В Relay Satellite (95°) and 2-way audio/video comm check with ШСС smart station [АРМ]
- Powered Ascent Utility Locker Hardware Installation
- Receiving files from ШСС MDM to RSS1
- DOSIS Passive Detector Pouch retrieval
- Installation of the DOSIS 3D passive detector packs in Columbus and photodocumentation.
- EHT Tissue Chamber Media Change
- Test comm sessions via Luch-5В Relay Satellite (95°) and 2-way audio/video comm check with ШСС smart station [АРМ]
- Closeout ops after comm sessions via Luch-5V (95°) relay satellite
- On MCC Go Turn off camcorder, TV system monitoring equipment, close applications
- LSG-Front Glove-Swap
- Restow Dragon Vestibule Outfitting Kit (VOK)
- Polar Samples to Cold Stowage
- PCG-10 Ambient Stow
- PCG-10 Hardware Photography
- Environmental Health System (EHS) Total Organic Carbon Analyzer (TOCA) Sample Data Record
- LSG Primary Crew Restraint Fold
- LSG Work Volume Stow