ISS Daily Summary Report – 3/26/2020

Engineered Heart Tissues: The crew performed sample fixations on selected tissue chambers and performed cell culture media exchanges on the remaining tissue chambers. A Human iPSC-based 3D Micro-physiological System for Modeling Cardiac Dysfunction in Microgravity (Engineered Heart Tissues) assesses human cardiac function in microgravity. It uses 3D cultured cardiac muscle tissue that mimics the architecture and function of adult human cardiac muscle. A magnet-based sensor placed underneath the tissue culture chamber allows real-time, non-destructive analysis of the functional performance and maturation of the tissues in space, which is compared with those cultured on Earth.

BioFabrication Facility (BFF): The crew placed a test cassettes (without cells) into the BFF for a test print. Cleaning Syringes were swapped out after the prints and photos were taken. The BFF is dedicated to manufacturing human organs and tissues in space, primarily for use by patients on Earth. Besides printing tissue, the BFF also can help maintain the health of deep space exploration crews by producing food and personalized pharmaceuticals on demand.

RADI-N2 (Radiation Dosimetry Inside ISS-Neutrons): The crew retrieved the 8 dosimeters from the Columbus module and handed over the units to CDR for data downlink. The objective of this Canadian Space Agency investigation is to better characterize the ISS neutron environment, define the risk posed to the crew members’ health, and provide the data necessary to develop advanced protective measures for future spaceflight.  It’s been recognized that neutrons make up a significant fraction (10-30%) of the biologically effective radiation exposure in low-Earth orbit.  The bubble detectors used in the investigation are designed detect neutrons and ignore all other radiation. 

Completed Task List Activities:

  • Photo documentation Engineered Heart Tissue (EHT) operations within LSG.

Today’s Ground Activities:
All activities are complete unless otherwise noted.

  • BioFabrication Facility Test Print
  • EHT Tissue Chamber Media Change and Fix/Preservation
  • SM 8.11 loads

Look Ahead Plan

Friday, 3/27 (GMT 087)

  • Vascular Echo Blood Collection (CSA)
  • BFF hardware uninstall (NASA)
  • MVP2 Cell-03 Nutrient tube and waste tube replacement (NASA)
  • Food Acceptability (NASA)
  • ISS HAM pass (NASA)
  • POLAR desiccant swap (NASA)
  • Everywear App configuration on Ipads (ESA)


  • SpX-20 EVA Pre-pack
  • Regen ECLSS Operations 

Saturday, 3/28 (GMT 088)

  • SpX-20 Cargo Operations

Sunday, 3/29 (GMT 089)

  • Crew Off-Duty Day 

Today’s Planned Activities:
All activities are complete unless otherwise noted.

  • ECON-M. Observation and Photography
  • IMS update
  • Preparation of reports for Roscosmos website and social media
  • Reminder Portable O2 Monitor (POM) Reading
  • SCENARIY. Observation and photography of catastrophic events and their aftermath
  • Standard Measures Post-sleep Questionnaire
  • Reminder Probiotics Fecal Operations
  • CSA Generic Frozen Blood Collection
  • LSG Work Volume Deploy
  • EHT MELFI Sample Retrieval Operation 1
  • EML Gas Valves Closing
  • BioFabrication Facility Test Print Without Cells Install 1
  • BioFabrication Facility MELFI Sample Retrieve
  • BioFabrication Facility Test Print Without Cells Install
  • Vozdukh Deactivation
  • Queso Service Pack Plan Review
  • Radi-N Detector Retrieval/Readout
  • MATRYOSHKA-R. Handover of BUBBLE-dosimeter detectors from USOS
  • Radiation Dosimetry Inside ISS-Neutrons Hardware Handover
  • MATRYOSHKA-R. BUBBLE-dosimeter gathering and measurements
  • Activation of Laptop RS2
  • LSG Primary Crew Restraint Unfold
  • EHT Tissue Chamber Media Change and Fix/Preservation
  • SSC2 Reload from Load Image Server
  • EHT MELFI Sample Insertion Operations Session 1
  • EHT MELFI Sample Insertion Operations Session 2
  • BioFabrication Facility Cleaning Syringe Install
  • Deactivation of RS3 Laptop
  • Vacuum Cleaning of Dust collectors ПC1, ПС2 filter cartridges in FGB (panels 203, 403)
  • Ultrasound 2 HRF Rack 2 Power On
  • Cleaning FGB ЦВ1 Circulation Fan Protective Screen
  • Health Maintenance System (HMS) Ultrasound 2 Scan
  • Activation of Vozdukh Atmosphere Revitalization Subsystem [СОА]
  • RS2 Laptop-КЦП2-ЦВМ-ТВМ Commanding & Data Transmission Test
  • BioFabrication Facility Test Cassette Removal
  • Regenerative Environmental Control and Life Support System (ECLSS) Recycle Tank Drain
  • BioFabrication Facility Historical Photo 2
  • CSA Generic Frozen Blood Collection Hardware Setup
  • Inventory Management System (IMS) Conference
  • LSG Primary Crew Restraint Fold
  • LSG Work Volume Stow
  • Regenerative Environmental Control and Life Support System (RGN) Wastewater Storage Tank Assembly (WSTA) Fill