ISS Daily Summary Report – 2/16/2021


Three-dimensional Microbial Mapping of ISS Environment (3DMM): The crew performed surface collections in PMM and the Airlock.  Surfaces in the ISS contain microbes and associated biomolecules excreted by these microorganisms.  3DMM uses DNA sequencing and other analyses to construct a 3D map of bacteria and bacterial products throughout the Station.  The team also plans to characterize how these microbes respond at a molecular level to specific stress conditions, including altered gravity and atmospheric composition.

Asian Herb in Space:  The crew set the plant chamber under the GLA lights and watered the seeds.  Microgravity affects the growth of plants and understanding the nature of these changes is important for future missions that will rely on plants for food.  Asian Herb in Space studies fast-growing plants used for traditional medicine and flavoring food, examining differences in their aroma that may result from microgravity-related cellular changes.  This investigation benefits future plant growth efforts in space, and provides new information on the formation of aroma compounds in common herbs.

Astrobee:  The crew had a conference with the Payload Developer prior to the start of Soundsee operations.  Astrobee is made up of three free-flying, cube-shaped robots which are designed to help scientists and engineers develop and test technologies for use in microgravity to assist astronauts with routine chores and give ground controllers additional eyes and ears on the space station.  The autonomous robots, powered by fans and vision-based navigation, perform crew monitoring, sampling, logistics management, and accommodate up to three investigations.

Confined Combustion:  The crew installed the Confined Combustion hardware in the MSG then conducted a flame test on one sample.  Confined Combustion examines the behavior of flame as it spreads in differently-shaped confined spaces in microgravity.  Flame spread observations are translated into mathematical models for use in understanding the results and applying them to areas with different shapes.

WetLab-2 One-Step Gene Sampling Tool:  The crew set up hardware for Wet Lab-2 One-Step Gene Sampler Operations then performed mRNA expression analysis on a radish harvested from Plant Habitat.  One-Step Gene Sampling Tool to Improve the ISS Bioanalytical Facility (One-Step Gene Sampling Tool) tests a technology that collects ribonucleic acid (RNA) directly from tissue.  It enables faster genetic analysis of multiple samples without the need to destroy the specimen, as traditional technology requires.  These brief and repeatable screening experiments can help researchers make more informed decisions regarding when to terminate plant or animal growth investigations and extract the full genetic library.

SoundSee:  The crew installed SoundSee onto an Astrobee Free-Flyer, then performed checkout of SoundSee.  The crew was able to perform two out of the 4 recordings planned.  Investigation of Deep Audio Analytics on the International Space Station (SoundSee Mission) tests monitoring of the acoustic environment using an audio sensor on Astrobee, a mobile robotic platform aboard the space station.  Microphones collect acoustic information, and the Astrobee determines the sensor’s position.  The system can detect anomalies in the sound of components inside a machine, providing autonomous monitoring of the health of infrastructure such as life support and exercise equipment.


Cygnus Robotic on Board Trainer (RoBOT): FE-10 and FE-12 set up the RoBOT and completed a proficiency training session.

Food Consolidation: The crew used Stow Track to consolidate food stowage. This will open up available space on the ISS.

Urine Processing Assembly (UPA) Recycle Tank: The crew R&Rd the UPA recycle tank as part of nominal scheduled maintenance. The vent adapters were R&Rd as well.

Centerline Berthing Camera System (CBCS): The crew deployed and checked out the CBCS at Node 1 Nadir to support future Common Berthing Mechanism (CBM) mating operations.

Completed Task List Activities:

  • ARED spreadsheet recording
  • Astrobee OBT review
  • Dragon Crew-1 debrief
  • WHC urine hydraulic components R&R
  • JAXA EPO audit
  • JAXA UN video
  • Vascular Echo ultrasound
  • Wanted poster Hunch tape dispenser
  • PWD circuit breaker reset
  • WHC KTO replace

Today’s Ground Activities:
All activities are complete unless otherwise noted.

  • Payloads ops support
  • Cygnus OBT RoBOT support
  • CBCS install/checkout support
  • C2V2 activation

Look Ahead Plan

Wednesday, February 17 (GMT 048)

  • Antimicrobial Coatings Touch, Combustion Integrated Rack Manifold #2 & #4 Bottle Replacement, ISS Experience Solid State Drive Changeout, Plant Water Management-Soil Prime and Operations, Standard Measures Post-sleep Questionnaire, Vascular Echo 13-Hour Blood Pressure Monitoring Initialization Alternate


  • OBT Cygnus offset grapple
  • EVA REBA powered hardware checkout
  • EVA  HD EMU camera assembly install
  • OBT Cygnus RoBOT self study

Thursday, February 18 (GMT 049)

  • AstroRad Vest Survey 2, Confined Combustion Test Operations, ISS Experience EVA Z-Cam Checkout, Plant Water Management Battery Exchange, Standard Measures Pre-sleep Questionnaire, Vascular Echo 13-Hour Blood Pressure Monitoring Conclude Alternate


  • EVA Z-cam checkout
  • EVA EMU resize
  • Cygnus cargo transfer review 

Friday, February 19 (GMT 050)

  • 3DMM Node 3 Sample Collection, Antimicrobial Coatings Touch, Airborne Particulate Monitor Status Check, DOSIS Main Box LED Check, Food Acceptability Survey, ISS Experience Recording, Packed Bed Reactor Experiment Historical Documentation Photos, SAMS CU HD Swap Load, Standard Measures Post-sleep Questionnaire, Time experiment science


  • Cygnus RoBOT OBT
  • Microbial monitoring sample collect

Today’s Planned Activities:
All activities are complete unless otherwise noted.

  • Three Dimensional Microbial Monitoring (3DMM) PMM Sample Collection
  • Total Organic Carbon Analyzer (TOCA) Water Recovery System (WRS) Sample Analysis
  • USB Flash Drive to be plugged into EICL
  • Capillary Structures Hardware Consolidate
  • Countermeasures System (CMS) Treadmill 2 (T2) Exercise Session
  • XF305 Camcorder Setup
  • Asian Herb Set Up
  • Solidification Using Baffles in Sealed Ampoules Hardware Stow
  • Cell Biology Experiment Facility Left (CBEF-L) Plant Exp Unit Detachment
  • Columbus Cabin photo audit
  • 3DMM MELFI Sample Insertion
  • Columbus Starboard EndCone photo audit
  • Astrobee Dock Power Cycle
  • Regenerative Environmental Control and Life Support System (ECLSS) Urine Processor Assembly (UPA) Brine Filter Changeout
  • Astrobee PREP
  • Robotic Workstation (RWS) Setup
  • USB Flash Drive to be removed from EICL and inserted in ICF
  • On-board Training (OBT) Cygnus Robotics Onboard Trainer (ROBoT) Session 1
  • Wet Lab-2 One-Step Gene Sampler MELFI Sample Retrieve
  • Wet Lab-2 One-Step Gene Sampler Hardware Setup
  • Confined Combustion Hardware Setup
  • Wet Lab-2 One-Step Gene Sampler MELFI Sample Retrieve
  • Wet Lab-2 One-Step Gene Sampler Sample Process
  • Preparing and installing thermostat «KASKAD» at the experiment location; connecting to onboard power
  • Vacuuming behind panels 405 and 406 in MRM1
  • Robotics Work Station (RWS) Display and Control Panel (DCP) Checkout
  • Environmental Health System (EHS) Total Organic Carbon Analyzer (TOCA) Sample Data Record
  • Vestibule Outfitting Kit Equipment Gather
  • Wet Lab-2 One Step Gene Sampler MELFI Sample Insertion into a Box Module
  • Regenerative Environmental Control and Life Support System (ECLSS) Recycle Tank Drain
  • Wet Lab-2 One-Step Gene Sampler SSC Cleanup [Deferred]
  • VZAIMODEISTVIE-2. Experiment run
  • Food Consolidation
  • Confined Combustion Test Operations
  • Countermeasures System (CMS) Treadmill 2 System (T2) Monthly Inspection
  • Recycle Tank Remove and Replace
  • Wet Lab-2 One-Step Gene Sampler Closeout [Deferred]
  • Astrobee Crew Conference
  • Astrobee Stowage Clear
  • Cygnus Centerline Berthing Camera System (CBCS) and Vestibule Closeout Equipment Gather
  • SOUNDSEE Installation and Checkout
  • ISS HAM Radio Power Down in Service Module
  • ISS HAM Kenwood Radio Power Down in Columbus
  • N1 Nadir Hatch to Unlatch Hardstop
  • Health Maintenance System (HMS) OCT2 Setup
  • Max Cycle Ergometer w/ Vibration Isolation & Stabilization (CEVIS) Portable PFS Partial Set Up
  • Astrobee Stowage Clear
  • Centerline Berthing Camera System (CBCS) Installation and Checkout
  • Dragon PCS Swap
  • Urine Transfer System Offload EDV Swap [Deferred]
  • Health Maintenance System (HMS) OCT2 Prep
  • Plant Water Management Battery Exchange
  • Metal Oxide (METOX) Regeneration Initiation
  • Health Maintenance System (HMS) – OCT2 Exam – Operator
  • Health Maintenance System (HMS) OCT2 Exam – Subject
  • USOS Window Shutter Close
  • Three Dimensional Microbial Monitoring (3DMM) Airlock Sample Collection
  • Health Maintenance System (HMS) OCT2 Prep
  • Health Maintenance System (HMS) – OCT2 Exam – Operator
  • Health Maintenance System (HMS) OCT2 Exam – Subject
  • Recycle Tank Fill Part 3 [Deferred]
  • Spectrum Relocation
  • 3DMM MELFI Sample Insertion
  • Health Maintenance System (HMS) OCT2 Stow