ISS Daily Summary Report – 12/28/2021


Advanced Plant EXperiment-07 (APEX-07):  The crew configured both Veggie Facilities for initial APEX-07 operations, preparation, and insertion of thirty Petri Plates per Veggie facility.  APEX-07 examines how changes in gravity and other environmental factors associated with spaceflight affect plants at the level of gene expression.  Previous research shows that microgravity conditions during spaceflight affects which genes turn on or off, which proteins are present and in what amounts, and the modifications made to those proteins.  All of these processes are controlled by RNA, and this investigation analyzes the role of RNA regulation on gene expression during spaceflight in both roots and shoots of plants.

Cytoskeleton:  Cytoskeleton Experiment Containers were installed on the Biolab Rotor.  In vitro cultures of mammalian cells react specifically to microgravity, but there is no solid scientific evidence available about how and why.  The Involvement of Rho family GTPases in gravity perception and reaction (Cytoskeleton) investigation attempts to acquire new information about the events that are happening inside a mammalian cell, with the working hypothesis that the microgravity environment affects the function of RhoGTPases and the cellular signaling that they control.  RhoGTPases are signaling molecules residing under the Rho family of proteins, known to be involved as “molecular switches”, in the control of cell proliferation, apoptosis (programmed cell death), gene expression (flow of genetic information from gene to protein), and cytoskeletal organization.

Electro-static Levitation Furnace (ELF):  A crewmember replaced the AC Dry Vacuum Cleaner Debris Bag in support of Electrostatic Levitation Furnace sample removing.  Lost and broken samples from the experiment volume were removed and stowed in Ziploc bags for return.  ELF is an experimental facility designed to levitate, melt, and solidify materials by containerless processing techniques using the electrostatic levitation method.  With this facility, thermophysical properties of high temperature melts can be measured and solidification from deeply undercooled melts can be achieved.

NanoRacks Platforms:  Imagery was taken of Platform-2 Module-79.  NanoRacks Platforms is a multipurpose research facility on board ISS. NanoRacks Platforms supports NanoRacks Modules in the CubeSat form factor by providing power and data transfer capabilities to operate investigations in microgravity.

P&G Telescience Investigation of Detergent Experiments (PGTIDE):  A crewmember took imagery of PGTIDE samples.  PGTIDE studies the effectiveness of stain removal ingredients in Tide To Go Pens and Tide To Go Wipes in microgravity.  The investigation also examines any changes in physical appearance of detergent and in stability and performance of enzymes in detergent formulations caused by exposure to microgravity. Results could support development of detergent systems for Artemis, Mars, and other future space missions where crews need the ability to launder clothing.


Temperature and Humidity Control (THC) Oxygen Regeneration System (OGS) Avionics Air Assembly (AAA) Flow Measurement: Today the crew recorded airflow measurements of the OGS rack AAA using the Velocicalc tool as part of regular maintenance activities being performed this week. The OGS AAA provides cooling to the Node 3 system rack.

Extravehicular Activity (EVA) Mobility Unit (EMU) Activities: Crewmembers reorganized an EVA Cargo Transfer Bag (CTB), which consisted of inspecting plastic bags and ensuring all hardware is contained in the plastic bags, and then photographing the contents. Crew also terminated a charge begun yesterday on two Li-Ion Batteries using the EVA Battery Operations Terminal and removed the batteries from the Battery Stowage Compartment. These batteries are part of the Rechargeable EVA Battery Assembly (REBA).

Completed Task List Activities:

  • None

Today’s Ground Activities:
All activities are complete unless otherwise noted.

  • MSS Powerdown/Powerup
  • THC OGS AAA Flow Measurement
  • SPDM MSC Transfer
  • Lab ITCS LTL Repressurization

Look Ahead Plan

Wednesday, December 29 (GMT 363)

  • 4 Bed CO2 Scrubber samples (NASA)
  • Food Acceptability (NASA)
  • MSG facility power down (NASA)
  • Ring Shear Drop stow (NASA)
  • Thermal Amine Samples (NASA)


  • Transfer Crew Dragon Cargo Ops
  • EHS PWD Sample Collect
  • EVA SLE Training
  • WHC Manual Fill
  • EHS TOCA WRS Sample Analysis
  • EHS Coliform Water Processing
  • EMU Swap
  • EMU SLE Rotation
  • COL ITCS Water Sample
  • IFM Node 1 Linear Diffuser Cleaning
  • Node 2 MTL Return to Ground Sampling
  • Node 2 LTL Return to Ground Sampling
  • Handover of Increment Crew
  • EMU 3013 Config
  • EDAR Installation
  • Emergency Mask OBT

Thursday, December 30 (GMT 364)

  • Cytoskeleton Deinstall (ESA)
  • Food Physiology Brief (NASA)
  • RR-18 Hab restock access clean and ops (NASA)


  • Regenerative ECLSS Recycle Tank Drain/Fill
  • Transfer Crew Dragon Cargo Ops
  • Russian EVA Tool Gather
  • IMS Conference
  • SSC 10 and 20 Swap
  • IFM WHC UR and IF R&R

Friday, December 31 (GMT 365)

  • Cytoskeleton setup and Experiment container install (ESA)
  • MISSE MSC removal and stow for return (NASA)
  • T2AR OBT, inspect and questionnaire (NASA) 


  • EHS – Coliform Water Sample Analysis
  • Water Recovery System CWC-Iodine Fill
  • Transfer Crew Dragon Cargo Ops
  • S&M JOTI Gather & JOTI Removal

Today’s Planned Activities:
All activities are complete unless otherwise noted.

  • HRF Generic Urine Collection Male
  • HRF Generic MELFI Sample Retrieval and Insertion Operations
  • APEX-07 Light Setup
  • HRF Generic HRF Centrifuge Setup and Frozen Blood Collection Subject
  • HRF Generic HRF Centrifuge Frozen Blood Collection Operator
  • HRF Generic HRF Centrifuge Frozen Blood Collection Configure and Run
  • Multi-use Variable-g Platform Desiccant Replace
  • ISS Emergency Soyuz 749 Descent OBT. Tagup with specialists
  • HRF Generic HRF Centrifuge Frozen Blood Collection Spin Conclude
  • Phospho-Aging Sample MELFI Retrieval and Insertion
  • HRF Generic HRF Centrifuge Frozen Blood Collection Conclude and Stow
  • APEX-07 Hardware Gather #1
  • Electrostatic Levitation Furnace (ELF) Item Gathering
  • Electrostatic Levitation Furnace (ELF) AC Dry Vaccum Cleaser Bag R&R
  • APEX-07 Iceberg Sample Removal
  • XF705 Camcorder Setup
  • APEX-07 Science Insertion
  • Electrostatic Levitation Furnace (ELF) Sample Cartridge Removal
  • RJ45 Connector Clip Replacement
  • EasyMotion Suit stow
  • Electrostatic Levitation Furnace (ELF) Sample Cartridge Cleaning
  • Med Reservoir retrieval from Biolab Glovebox
  • Turning off camcorder, TV system controls, closing applications
  • Cytoskeleton LSG Preperation and EC Hardware Staging
  • Electrostatic Levitation Furnace (ELF) Sample Holder Change
  • Wanted Poster for Oxygen Generation System (OGS) Velocicalc Adapter
  • Gigastor Hard Drive Swap
  • Air Quality Monitor USB Fan Deploy
  • Air Quality Monitor Power On
  • HRF Rack 2 PC Software Integrated Build Load Installation Preparation
  • EVA Cargo Transfer Bag (CTB) Reorganize
  • NanoRacks Module-48 Imagery
  • Countermeasures System (CMS) Advanced Resistive Exercise Device (ARED) Pulley Inspection
  • Temperature and Humidity Control (THC) Oxygen Generation System (OGS) Avionics Air Assembly (AAA) Flow Measurement
  • Cytoskeleton EC Zero G RNAlater Assembly
  • Transfer Crew Dragon Cargo Operations
  • Cold Stowage Double Coldbag Icebrick Stow
  • PGTIDE Detergent Stability Study Big Picture Words Review
  • Cytoskeleton LSG Cleanup and Prep
  • PGTIDE Maintenance Work Area Preparation
  • Cytoskeleton Polar Sample Removal
  • Cytoskeleton LSG Transport Container Insertion
  • PGTIDE Cupola Photography
  • PGTIDE Detergent Stability Study Sample Photo Operations
  • Cytoskeleton EC Zero G PFA Assembly
  • Columbus cabin cargo consolidation
  • EC installation in Biolab Incubator
  • HRF Generic Urine Collection Stow
  • LSG Primary Crew Restraint Fold/Unfold
  • Handover of Increment 66 Crew
  • iPad signal strength characterization and log gathering
  • EVA Battery Operations Terminal Charge Terminate
  • Extravehicular Activity (EVA) Battery (BATT) Remove
  • Video recording of greetings
  • NanoRacks Module-79 Imagery
  • LSG Work Volume Stow