ISS Daily Summary Report – 3/21/2023


Cardinal Heart 2.0: A media change was performed for Samples 1B and 1A. A previous investigation showed that four weeks of microgravity exposure caused significant changes in heart cell function and gene expression that could lead to long-term damage or cardiac muscle atrophy. Effect of Microgravity on Drug Responses Using Heart Organoids (Cardinal Heart 2.0) uses heart organoids to test whether clinically approved drugs reduce these microgravity-induced changes in heart cell function. Results could support development of effective drug combinations to improve the health of astronauts and patients on Earth.

Life Science Glovebox (LSG): The front gloves were swapped out from the LSG facility. The Life Sciences Glovebox (LSG) is a sealed work area that accommodates life science and technology investigations in a “workbench” type environment. Due to its larger size design, two crew members can work in the LSG simultaneously.

Rhodium DARPA Biomanufacturing 01: Science Chamber Sample 1 was removed from the Rhodium Variable Gravity Simulator facility and placed into cold stowage and Sample 2 was installed into the Gravity Simulator Facility. Efficient and Resilient Biomanufacturing in Variable Gravity (Rhodium DARPA Biomanufacturing 01) characterizes the effect of different levels of gravity on the production of therapeutics and nutraceuticals from bacteria and yeast. Prior spaceflight studies show that microgravity causes changes in microbe cell growth, cell morphology, and metabolic activity that affect biomanufacturing performance. Results could inform strategies to improve biomanufacturing in space and provide for humans on future missions.

Space Health: The Bio-Monitor garment was changed out as part of the 48-hr data collection session. The Autonomous Health Monitoring for Adaption Assessment on Long Range Missions Using Big Data Analytic (Space Health) investigation utilizes the Bio-Monitor system for physiological monitoring before, during, and after a mission to the International Space Station to assess the effect of space travel on heart health. The Artemis analytical platform is used to provide automated analysis of the cardiovascular system in order to develop a near real-time assessment tool. The potential use of the Bio-Monitor system and the Artemis platform for future space missions is evaluated.

Veg-05: The plants growing in the two Veggie facilities were inspected, watered as needed, and photographed. The Pick-and-Eat Salad-Crop Productivity, Nutritional Value, and Acceptability to Supplement the ISS Food System (Veg-05) investigation is the next step in efforts to address the need for a continuous fresh-food production system in space.


Potable Water Dispenser (PWD) Sample Collection: The crew performed periodic Environmental Health System (EHS) water sampling by collecting water samples from the PWD for in-flight and post-flight analysis. In-flight samples were used to perform a Total Organic Carbon Analyzer (TOCA) analysis that measures the amount of organic constituents in the potable water. The crew also tested the water samples for the presence of Coliform bacteria utilizing a Coliform test bag. The post flight samples will return on SpX-27 for additional ground analysis. These tests are used to determine if the drinking water remains safe for crew consumption.

Transfer SpX-27 Cargo Operations: The crew transferred payloads from the SpX-27 cargo vehicle to the ISS. The crew will continue removing the more than 6,200 pounds of cargo launched on SpX-27 and then will begin to load SpX-27 for return.

Completed Task List Activities:

  • None

Today’s Ground Activities:
All activities are complete unless otherwise noted.

  • SSRMS Walkoff
  • JEMRMS Small Fine Arm (SFA) Extrantion form SSE
  • HRF1 Rack Powerup Command

Look Ahead Plan

Wednesday, March 22 (GMT 081)

  • Cardinal Heart 2.0 Microscopy (NASA)
  • DREAMS Headband Setup and Don (ESA)
  • HRF Collections (NASA)
  • HRF Veggie POMS question (NASA)
  • Space Health Bio-Monitor Closeout and Stow, questionnaire (CSA)


  • Dragon Cargo Operations
  • WHC Manual Fill
  • T2 Monthly Inspection

Thursday, March 23 (GMT 082)

  • Cardinal Heart 2.0 Hab Fixation (NASA)
  • DREAMS Headband Doff and Setup (ESA)
  • HRF Collections (NASA)
  • LSG Front Glove Swap and Work Volume Stow (NASA)
  • Lumina Reboot and Data Transfer (ESA)
  • POLAR Desiccant Swaps (NASA)
  • SoFIE GEL HW Replacement (NASA)
  • Space Health Bio-Monitor Setup for 48hr Session (CSA)
  • Standard Measures Collection Setup (NASA)
  • Veg-05 Rootmat Fill and Check (NASA)


  • Dragon Cargo Operations
  • EMER Air Supply Activity
  • Smart Watch Charge for SpX Crew Dragon

Friday, March 24 (GMT 083)

  • Astrobee Zero-Robotics Tech Demo (NASA)
  • BioFabrication Facility Cassette Insert (NASA)
  • DREAMS Headband Doff and Data Transfer and Stow (ESA)
  • Food Physiology Diet Brief (NASA)
  • FRIDGE Card Exchange (NASA)
  • MISSE-17 MSC Install (NASA)
  • Space Health Bio-Monitor Changeout and Questionnaire (CSA)
  • Standard Measures Collections (NASA)
  • Vascular Aging 13-Hr Blood Pressure Monitor (CSA)
  • Veg-05 Harvest-Final (NASA)


  • Dragon Cargo Operations
  • Recycle Tank Drain

Today’s Planned Activities:
All activities are complete unless otherwise noted.

  • Bio-Monitor Breathing Volume Calibration
  • Bio-Monitor ECG Electrode Substance Reapply
  • Bio-Monitor Wearables Change Out and Stow
  • Cardinal Heart 2.0 Media Change and Sampling
  • Cardinal Heart 2.0 MELFI Sample Insertion
  • Acoustic Monitor Battery Swap
  • Acoustic Monitor Setup for Crew Worn Measurements
  • Coliform Water Processing
  • Potable Water Dispenser (PWD) Sample Collect and Analysis
  • On-orbit Hearing Assessment (OOHA) with Kuduwave Software Setup and Test
  • EveryWear Evaluation
  • Ultrasound 2 Prep, Scan, and Exam
  • Ultrasound 2 HRF Rack 1 Power On
  • HRF Generic MELFI Sample Retrieval and Insertion
  • HRF Generic Sample Collection
  • LSG-Front Glove-Swap
  • LSG Primary Crew Restraint Fold and Unfold
  • Public Affairs Office (PAO) Event – Lab
  • Rhodium DARPA Biomanufacturing-01 Iceberg Sample Insert and Removal
  • Rhodium DARPA Biomanufacturing-01 Rhodium Science Chambers 0001 Remove from VGS
  • Rhodium DARPA Biomanufacturing-01 Rhodium Science Chambers 0002 VGS Install and Activate
  • Space Health Experiment Questionnaire
  • Standard Measures Post-Sleep Questionnaire
  • VEG-05 Plant Check/Water
  • Transfer Cargo Dragon Cargo Operations