Eye and Brain Study, Fluid Physics Research After Cosmonaut Spacewalk

Cosmonaut Sergey Prokopyev's helmet camera captures Earth views and cosmonaut Dmitri Petelin at work during the April 18 Russian spacewalk to install a radiator on the Nauka science module. Credit: NASA TV
Cosmonaut Sergey Prokopyev’s helmet camera captures Earth views and cosmonaut Dmitri Petelin at work during the April 18 Russian spacewalk to install a radiator on the Nauka science module. Credit: NASA TV.

Human research and fluid physics were the main science experiments taking place aboard the International Space Station on Wednesday for four Expedition 69 astronauts. The three cosmonauts aboard the orbital outpost slept in following an overnight spacewalk to move and install hardware.

The latest space biology experiment taking place on the station explores how living long-term in weightlessness affects an astronaut’s eyes and brain. The lack of gravity causes blood and cerebrospinal fluid to shift toward the head creating ocular and cranial pressure. NASA astronauts Stephen Bowen and Woody Hoburg studied that phenomena Wednesday morning and ways to offset the space-caused symptoms by collecting their blood pressure measurements and scanning their chests with the Ultrasound 2 device for the ISAFE investigation.

Afterward, Bowen peered at biological samples inside the Confocal Microscope testing its operations using fluorescence imaging techniques. Hoburg joined UAE (United Arab Emirates) Flight Engineer Sultan Alneyadi setting up hardware to kick off the CapiSorb Visible Systems fluid physics study. That experiment investigates the potential of using a liquid-based carbon dioxide removal system to promote more efficient space-based solutions and advanced Earth-bound applications.

The three astronauts, together with NASA Flight Engineer Frank Rubio, also spent a portion of Wednesday afternoon reviewing procedures and practicing robotic maneuvers for a spacewalk planned for April 28. Bowen and Alneyadi are scheduled to spend about six-and-a-half hours in the vacuum of space upgrading the orbital outpost’s power generation system. Space station managers will discuss the upcoming spacewalk live on NASA TV’s app and the agency’s website at 2 p.m. EDT on Monday.

The third spacewalk of the year was completed overnight after Commander Sergey Prokopyev and Flight Engineer Dmitri Petelin installed a radiator on the Nauka science module. The duo wrapped up the seven-hour and 55-minute logistics spacewalk at 5:35 a.m. on Wednesday, with assistance from Roscosmos Flight Engineer Andrey Fedyaev who operated the European robotic arm (ERA) with the radiator in its grip.

The trio from Roscosmos will have a long sleep period before returning to a normal shift on Thursday. Prokopyev and Petelin will then spend the day cleaning their Orlan spacesuits and stowing their spacewalking tools in the Poisk module. Fedyaev will return the ERA to its stowage position on Nauka then power it down.


Learn more about station activities by following the space station blog, @space_station and @ISS_Research on Twitter, as well as the ISS Facebook and ISS Instagram accounts.

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SpaceX Cargo Dragon Splashes Down, Returning Science to Earth for NASA

The Solid Fuel Ignition and Extinction (SoFIE) Growth and Extinction Limits (GEL) experiment aboard the International Space Station (ISS) studies flame growth and extinction in an effort to improve fire safety in space. This image shows a sequence of snapshots taken about 3 seconds apart. Gel samples from the investigation are returning to Earth for further analysis on the SpaceX CRS-27 cargo Dragon spacecraft.
The Solid Fuel Ignition and Extinction (SoFIE) Growth and Extinction Limits (GEL) experiment aboard the International Space Station (ISS) studies flame growth and extinction in an effort to improve fire safety in space. This image shows a sequence of snapshots taken about 3 seconds apart. Gel samples from the investigation are returning to Earth for further analysis on the SpaceX CRS-27 cargo Dragon spacecraft.

SpaceX’s uncrewed Dragon cargo spacecraft splashed down at 4:58 p.m. EDT Saturday, April 15, off the coast of Tampa, Florida, marking the return of the company’s 27th contracted cargo resupply mission to the International Space Station for NASA. The spacecraft carried approximately 4,300 pounds of valuable scientific experiments and other cargo back to Earth.

Some of the scientific investigations that Dragon is carrying include:

  • Space tomato harvest: Samples from the Pick-and-Eat Salad-Crop Productivity, Nutritional Value, and Acceptability to Supplement the International Space Station Food System (Veg-05) experiment will be returning to Earth for analysis. Astronauts grew dwarf tomatoes in the station’s Veggie miniature greenhouse and performed three harvests at 90, 97, and 104 days. They froze tomatoes, water samples, and swabs of the growth hardware to examine the effects of light quality and fertilizer on fruit production, microbial safety, and nutritional value. The ability to grow plants in space for fresh food and an improved crew living experience is important for future long-duration missions. The hardware could be adapted for use on Earth to provide fresh produce for those without access to gardens and as horticultural therapy for older people and people with disabilities.
  • Growing higher quality crystals: Hicari, an investigation from JAXA (Japan Aerospace Exploration Agency), tested a growth method and produced crystals of a silicon-germanium (SiGe) semiconductor using the Japanese Experiment Module-Gradient Heating Furnace (JEM-GHF). This crystal growth method could support development of more efficient solar cells and semiconductor-based electronics. The space-produced crystals are returning to Earth for analysis.
  • Analyzing aging arteries: Astronauts can experience accelerated arterial wall stiffening and thickening after six months in space, and a daily session of aerobic exercise alone may not be sufficient to counteract these effects. Vascular Aging, an investigation from CSA (Canadian Space Agency), monitors these changes using artery ultrasounds, blood samples, glucose tolerance tests, and wearable sensors. Results could help identify and assess risk to astronaut cardiovascular health and point to mechanisms for reducing that risk. For the aging population on Earth, understanding the mechanisms behind arterial stiffness could provide insight to guide prevention and treatment. Blood samples collected for the investigation are returning to Earth for analysis.
  • Fire safety: Solid Fuel Ignition and Extinction – Growth and Extinction Limit (SoFIE-Gel) studies burning in microgravity, including how fuel temperature affects material flammability. The investigation could improve safety of crew members on future missions by increasing understanding of early fire growth behavior, informing selection of fire-resistant spacecraft cabin materials, validating flammability models, and helping to determine optimal fire suppression techniques. Studying flames in space without the complications of buoyancy also helps improve computer models of combustion for terrestrial applications. Gel samples from the investigation are returning to Earth for further analysis.

Learn more about station activities by following the space station blog, @space_station and @ISS_Research on Twitter, as well as the ISS Facebook and ISS Instagram accounts.

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SpaceX Cargo Dragon Departs the Space Station

A SpaceX cargo Dragon is seen as it departs the International Space Station on April 15, 2023.
A SpaceX cargo Dragon undocked from the International Space Station at 11:05 a.m. EDT on April 15, 2023, as the station was flying over the Indian Ocean. It will return nearly 4,300 pounds of scientific samples and hardware for NASA.

Following commands from ground controllers at SpaceX in Hawthorne, California, Dragon undocked at 11:05 a.m. from the forward port of the station’s Harmony module. At the time of undocking the station was flying over the Indian Ocean.

After re-entering Earth’s atmosphere, the spacecraft will make a parachute-assisted splashdown off the coast of Florida on Saturday, April 15. NASA will not broadcast the splashdown, but updates will be posted on the agency’s space station blog.

Dragon arrived at the space station March 16 as SpaceX’s 27th Commercial Resupply Services mission for NASA, delivering more than 6,000 pounds of research investigations, crew supplies, and station hardware. It was launched March 14 on a SpaceX Falcon 9 rocket from Launch Complex 39A at Kennedy.


Learn more about station activities by following the space station blog, @space_station and @ISS_Research on Twitter, as well as the ISS Facebook and ISS Instagram accounts.

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NASA TV is Live for SpaceX Cargo Dragon Departure

A SpaceX Dragon resupply ship approaches the International Space Station carrying more than 6,200 pounds of science experiments, crew supplies, and other cargo on March 16, 2023.
A SpaceX Dragon resupply ship approaches the International Space Station carrying more than 6,200 pounds of science experiments, crew supplies, and other cargo on March 16, 2023.

Live coverage of the departure of SpaceX’s uncrewed Dragon cargo spacecraft from the International Space Station is underway on NASA Television, the agency’s website, and the NASA app.

Following commands from ground controllers at SpaceX in Hawthorne, California, Dragon will undock at 11:05 a.m. EDT from the forward port of the station’s Harmony module and fire its thrusters to move a safe distance away from the station.

After re-entering Earth’s atmosphere, the spacecraft will make a parachute-assisted splashdown off the coast of Florida on Saturday, April 15. NASA will not broadcast the splashdown, but updates will be posted on the agency’s space station blog.


Learn more about station activities by following the space station blog, @space_station and @ISS_Research on Twitter, as well as the ISS Facebook and ISS Instagram accounts.

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Crew Bioprints Cells, Prepares for Final Plant Harvest, and Conducts Robotics Operations With Students

NASA astronaut and Expedition 68 Flight Engineer Frank Rubio checks tomato plants growing inside the International Space Station for the XROOTS space botany study on Oct. 14, 2022. Credit: Koichi Wakata/Japan Aerospace Exploration Agency
NASA astronaut and Expedition 68 Flight Engineer Frank Rubio checks tomato plants growing inside the International Space Station for the XROOTS space botany study on Oct. 14, 2022. Credit: Koichi Wakata/Japan Aerospace Exploration Agency

The Expedition 68 crew members conducted space health experiments and prepared for the final plant harvest aboard the International Space Station while inspiring the next generation of explorers in a virtual robotics competition.

NASA Flight Engineer Woody Hoburg spent most of his day installing tissue cassettes for the BFF-Meniscus-2, an investigation to print and culture a meniscus using the BioFabrication facility aboard the space station. Crew members who experience musculoskeletal injuries on future deep space missions may benefit from the capability to bioprint tissue to promote recovery. The research could lead to the ability to print complex tissues and organs that may be used to treat patients on Earth.

NASA Flight Engineer Frank Rubio conducted the Astrobee-Zero Robotics operations, a programming competition where students earn the opportunity to control an autonomous flying robot and observe its performance aboard the space station. Finalists have their code downloaded by NASA to the Astrobee platform and observe its performance. Rubio also continued to transfer the 6,200 pounds of research hardware and supplies between the orbital outpost and the uncrewed SpaceX CRS-27, which arrived at the space station on March 16.

Afterward, Rubio spent the rest of his day preparing for the final harvest of the Veg-05 plants by collecting plant swabs and water samples to be stored in cold stowage for later use in research. The plant botany study is the next step in addressing the need for a food production system in space. The Vegetable Production System supplies crew members with a continuous source of fresh food and a tool for relaxation and recreation.

NASA Flight Engineer Stephen Bowen installed Materials International Space Station Experiment-17-Commercial hardware onto the Japanese Experiment Module airlock slide table. The investigation tests how the space environment affects the durability of materials and components, including 3D-printed polymers, thermal protection systems, spacecraft materials, biopellets made from dried microbes, paraffin wax thermal protection, and thin solar cells.

Flight Engineer Sultan Alneyadi from UAE (United Arab Emirates) removed the Dry-EEG Headband and synchronized the unit to the European Physiology Module laptop for data transfer. The sleep monitoring technology investigates astronauts’ sleep quality by measuring duration, sleep stages, heart rate, and the number of awakenings. Alneyadi then positioned the Astrobee free flyer in the Japanese Experiment Module and conducted software test runs.

Flight Engineer Andrey Fedyaev of Roscosmos continued to replace the condensate evacuation lines that carry away excess moisture from the cabin atmosphere. Meanwhile, Roscosmos cosmonauts Sergey Prokopyev and Dmitri Petelin prepared cargo to return in the Soyuz MS-22 spacecraft, slated to undock from the station’s Rassvet module on March 28.


Learn more about station activities by following the space station blog, @space_station and @ISS_Research on Twitter, as well as the ISS Facebook and ISS Instagram accounts.

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Crew Talk Space With Students, Investigate Fire Control, and Continue Heart Health and Cargo Return Activities

The big island of Hawaii and its two snow-capped volcanos, (from left) the active Mauna Loa and the dormant Mauna Kea, are pictured from the International Space Station as it orbited 260 miles above the Pacific Ocean on March 6, 2023.
The big island of Hawaii and its two snow-capped volcanos, (from left) the active Mauna Loa and the dormant Mauna Kea, are pictured from the International Space Station as it orbited 260 miles above the Pacific Ocean on March 6, 2023.

Since the earliest days of the International Space Station expeditions, student groups in schools, camps, museums, and planetariums have had the opportunity to talk with astronauts aboard the orbital laboratory about career choices and science activities. On Thursday, NASA Flight Engineer Woody Hoburg conducted an ISS Ham Radio (ARISS) session with Lana’i High and Elementary School, in Lana’i City, Hawaii.  Hoburg also bioprinted cells for the BFF-Meniscus-2, an investigation to print and culture a meniscus using the BioFabrication facility aboard the space station.

NASA Flight Engineer Frank Rubio replaced experiment samples in the Combustion Integrated Rack located in the U.S. Destiny module for the Solid Fuel Ignition and Extinction – Growth and Extinction Limit (SOFi) investigation. SOFi measures the amount of heating in a fuel sample to determine how fuel temperature affects material flammability in microgravity. Results could improve understanding of early fire growth behavior in space and help determine optimal fire suppression techniques.

Rubio also checked the Veg-05 plants and collected detached tomatoes to weigh. The plant botany study is the next step in addressing the need for a food production system in space. The Vegetable Production System supplies crew members with a continuous source of fresh food and a tool for relaxation and recreation.

NASA Flight Engineer Stephen Bowen spent his day wearing the Bio-Monitor garment and headband as part of a 48-hour session. The instrument is equipped with sensors to measure physiological parameters to assess the effect of space travel on heart health.

Flight Engineer Sultan Alneyadi from UAE (United Arab Emirates) injected the Cardinal Heart 2.0 with a preservative inside the Life Sciences Glovebox, a sealed work area in the space station where crew members perform developmental biology experiments. The investigation uses heart organoids to study the effects of clinical drugs on improving the function of heart cells exposed to microgravity.

Flight Engineer Andrey Fedyaev of Roscosmos continued to replace the condensate evacuation lines that carry away excess moisture from the cabin atmosphere. Meanwhile, Roscosmos cosmonauts Sergey Prokopyev and Dmitri Petelin prepared cargo to return in the Soyuz MS-22 spacecraft, slated to undock from the station’s Rassvet module on March 28.


Learn more about station activities by following the space station blog, @space_station and @ISS_Research on Twitter, as well as the ISS Facebook and ISS Instagram accounts.

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Crew Focuses on Experiments and Equipment to Maintain Health While in Space

The southern coast of Turkey on the Mediterranean Sea near Syria is pictured from the International Space Station as it orbited 264 miles above on Feb. 14, 2023.
The southern coast of Turkey on the Mediterranean Sea near Syria is pictured from the International Space Station as it orbited 264 miles above on Feb. 14, 2023.

The Expedition 68 crew members conducted experiments and maintained equipment aboard the International Space Station while activities for cargo transfers continued.

NASA Flight Engineer Frank Rubio performed microscopy and video recordings on eight BioCell tissue chambers for the Cardinal Heart 2.0 in the Life Sciences Glovebox. This investigation uses heart organoids to test whether clinically approved drugs reduce microgravity-induced changes in heart cell function. Rubio also removed the Advanced Resistive Exercise Device’s (ARED) cylinder flywheel and inspected the treadmill as part of monthly maintenance. The ARED exercises all major muscle groups while focusing on the primary resistive exercise: squats, deadlifts, and heel raises. Crew members exercise daily on ARED to maintain preflight muscle and bone strength during long periods in space.

Flight Engineer Sultan Alneyadi from UAE (United Arab Emirates) recorded a video of Cardinal Heart 2.0 tissue chambers. The investigation tests clinically approved pharmaceutical drugs to reverse the negative effects on heart cells and tissues caused by prolonged exposure to the space environment. Afterward, Alneyadi removed the Bio-Monitor garment and headband and synchronized the unit to the controller for data transfer. Alneyadi donned the Dry-EEG Headband overnight for sleep studies in space. Considering the central role of sleep in human behavior and health, sleep quality is a key factor for current and future exploration missions. The investigation monitors crew members’ quality of sleep by measuring duration, sleep stages, heart rate, and the number of awakenings.

Additionally, Rubio and Alneyadi spent the evening continuing to transfer the 6,200 pounds of research hardware and supplies between the space station and the uncrewed SpaceX CRS-27, which arrived at the orbital outpost on March 16.

Flight Engineer Andrey Fedyaev of Roscosmos continued to replace the condensate evacuation lines that carry away excess moisture from the cabin atmosphere. Meanwhile, Roscosmos cosmonauts Sergey Prokopyev and Dmitri Petelin prepared cargo to return in the Soyuz MS-22 spacecraft, slated to undock from the station’s Rassvet module on March 28.


Learn more about station activities by following the space station blog, @space_station and @ISS_Research on Twitter, as well as the ISS Facebook and ISS Instagram accounts.

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Crew Spends Day Investigating Biology and Continuing Cargo Operations

The northwestern coastal regions of Brittany and Normandy in France meet on the English Channel in this photograph from the International Space Station as it orbited 264 miles above on Feb. 12, 2023.
The northwestern coastal regions of Brittany and Normandy in France meet on the English Channel in this photograph from the International Space Station as it orbited 264 miles above on Feb. 12, 2023.

The Expedition 68 crew members are busy investigating biology and continuing to transfer the 6,200 pounds of research hardware and supplies between the International Space Station and the uncrewed SpaceX CRS-27, which arrived to the space station on March 16.

NASA flight engineer Frank Rubio conducted the Cardinal Heart 2.0 media change and sample collection operations in the Life Sciences Glovebox, a sealed work area in the space station where crew members perform developmental biology experiments. The investigation will test whether clinically approved drugs reduce microgravity-induced changes. Microgravity exposure can cause changes in heart cell function and gene expression that may lead to long-term damage or muscle atrophy.

NASA flight engineer Woody Hoburg took over the operations after participating in a cell biology experiment and collecting water samples from the potable water dispenser. The water recycling system reclaims wastewater into potable drinking water aboard the orbiting laboratory.

Flight Engineer Sultan Alneyadi from UAE (United Arab Emirates) installed rhodium science chambers in the variable gravity simulator for the Rhodium DARPA Biomanufacturing 01 investigation. Results may help reduce the costs of future missions by improving space biomanufacturing, the process of using microbes in the space station to produce materials and biomolecules like biopolymers, food, and pharmaceuticals.

Flight Engineer Andrey Fedyaev of Roscosmos spent the evening replacing the condensate evacuation lines that carry away excess moisture from the cabin atmosphere. Meanwhile, Roscosmos cosmonauts Sergey Prokopyev and Dmitri Petelin spent the day performing an eye ultrasound exam with a remote guide after gathering cargo items to return in the Soyuz MS-22 spacecraft, slated to undock from the station’s Rassvet module on March 28.


Learn more about station activities by following the space station blog, @space_station and @ISS_Research on Twitter, as well as the ISS Facebook and ISS Instagram accounts.

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Crew Spends Day Preparing for New Research and Operations Aboard the Space Station

This view of Mount Shasta in California was photographed from the International Space Station as it orbited 260 miles above on Feb. 12, 2023.
This view of Mount Shasta in California was photographed from the International Space Station as it orbited 260 miles above on Feb. 12, 2023.

Following the arrival of the uncrewed SpaceX CRS-27 resupply vehicle last week, the Expedition 68 crew continues transferring the 6,200 pounds of research hardware and supplies between the International Space Station and the cargo vehicle.

NASA Flight Engineer Frank Rubio spent his day participating in a cell biology experiment and replacing the Advanced Resistive Exercise Device cable arm rope to ensure proper tension in the cable-pulley system. Rope routing is critical for crew members to exercise in space, allowing crew members to experience load or resistance to help maintain muscle strength and mass during long periods in space.

NASA flight engineers Woody Hoburg and Stephen Bowen and Flight Engineer Sultan Alneyadi from UAE (United Arab Emirates) inserted ice bricks into the station’s Minus Eighty Degree Laboratory for ISS (MELFI) for temperature conditioning.  MELFI is a cold storage unit that maintains experiment samples at ultra-cold temperatures throughout a mission and supports a wide range of life science experiments by preserving biological samples (such as blood, saliva, urine, microbial or plant samples) collected aboard the space station for later return and analysis back on Earth.

Hoburg installed the Tanpopo-5 hardware and samples onto the slide table in the Japanese Experiment Module airlock. The investigation studies the possibility of the survival and growth of organisms in the space environment and on extraterrestrial planets, such as Mars. Hoburg also performed a hearing test with on-orbit hearing assessment headset and software, and took sound measurements using the acoustic monitor.

Alneyadi spent his day wearing the Bio-Monitor garment and headband for a 48-hour session. The instrument is equipped with sensors to measure physiological parameters to assess the effect of space travel on heart health.

Meanwhile, Roscosmos cosmonauts Sergey Prokopyev and Dmitri Petelin spent their day gathering cargo items to return in the Soyuz MS-22 spacecraft, slated to undock from the station’s Rassvet module on March 28.


Learn more about station activities by following the space station blog, @space_station and @ISS_Research on Twitter, as well as the ISS Facebook and ISS Instagram accounts.

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Dragon Cargo Transfer, Installations, and Eye Exams Cap Crew Week

The waxing gibbous Moon is pictured above Earth's horizon from the International Space Station as it orbited 260 miles above eastern China near the Yellow Sea coast on Feb. 2, 2023.
The waxing gibbous Moon is pictured above Earth’s horizon from the International Space Station as it orbited 260 miles above eastern China near the Yellow Sea coast on Feb. 2, 2023.

The Expedition 68 crew members wrapped up their week aboard the International Space Station by removing payloads for a resupply mission, installing equipment for microgravity research, and performing eye exams for a routine checkup.

NASA Flight Engineers Frank Rubio and Woody Hoburg and Flight Engineer Sultan Alneyadi from UAE (United Arab Emirates) made progress transferring supplies from the SpaceX cargo Dragon. Flight Engineer Stephen Bowen from NASA gathered hardware delivered by the spacecraft for the Heart-Tissue 2 study. The investigation will test whether clinically approved drugs reduce microgravity-induced changes in heart cells and tissues.

Outside of Dragon cargo operations, Rubio migrated double-cold bags for transporting samples from the station’s Microgravity Experiment Research Locker Incubators (MERLIN) to the Space Automated Bioproduct Laboratory (SABL). He also harvested tomatoes from the Veggie Vegetable Production System (Veggie) for the Veg-05 space botany study.

Hoburg performed installations to the Nanoracks Nanod, which provides power and data transfer capabilities for carrying out studies in microgravity. Additionally, he made configurations to the HAM radio used to communicate with students on Earth, cleaned vents and removed cartridges in air quality monitors, and performed an inspection of the station’s bathroom.

Bowen completed a training session that teaches astronauts docking and grappling techniques. He also exercised using the advanced resistive exercise device (ARED) and Cycle Ergometer with Vibration Isolation and Stabilization (CEVIS) device.

Meanwhile, Alneyadi performed installations to a research facility called ICE Cubes, which hosts different experiments in microgravity with the aid of small, modular containers that slot into a rack drawer. He also recorded a video for a diabetes study inside the facility. Near the end of the day, Alneyadi installed 24 Kubik experiment containers for the ESA (European Space Agency) – Biofilms investigation, which analyzes bacterial biofilm formation and the antimicrobial properties of different metal surfaces in space.

Planning ahead, the cosmonauts aboard the station met for a conference to prepare for next week’s tasks. Later, Flight Engineer Andrey Fedyaev of Roscosmos spent time arranging tools and cleaning smoke detectors. Near the end of the day, Cosmonauts Sergey Prokopyev and Dmitri Petelin completed a routine Optical Coherence Tomography (OCT) eye exam, which uses an imaging technique analogous to ultrasound imaging with light instead of sound.


Learn more about station activities by following the space station blog, @space_station and @ISS_Research on Twitter, as well as the ISS Facebook and ISS Instagram accounts.

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